Ruicheng Optoelectronics supplies high-end fluorescent filters
Fluorescent filters, also known as fluorescent filters, are widely used in various fields. Its main function is to separate and select the excitation and emission spectra of substances.
Fluorescent filters typically use a transparent glass substrate and are coated on both surfaces. The thickness of the anti reflection film will be selected as an integer multiple of half the wavelength, at which point the reflection superposition will be enhanced. The anti reflection thickness is a multiple of a quarter wavelength, and the superposition is enhanced at this time.
Fluorescent filters include various types of fluorescent filters, commonly used in microscopes to facilitate the observation of various fluorescent phenomena.
How to choose a fluorescent filter
The selection principle of fluorescence filters is to allow fluorescence (emitted light) to pass through the imaging end as much as possible and block excitation light to achieve high signal-to-noise ratio. Especially in the application of multiphoton excitation and total internal reflection microscopy, weak noise can cause significant interference to the imaging effect.
Usually, we study the transmission spectra of various parameters of fluorescent filters, but compared to the intensity of excitation light, fluorescence is often weak, so it is more necessary to refer to the blocking depth spectrum ODvalue to select fluorescent filters. Can intuitively reflect the shading characteristics of the filter.
The OD intersection point is an important criterion when selecting the excitation filter and the emission filter. Generally, ODvalue greater than 5 and ODvalue greater than OD6 are required to achieve better fluorescence signal-to-noise ratio (SNR).
Classification method of fluorescent filters:
According to different purposes, filters can be divided into TIRF filters, interference filters, total internal reflection filters, Raman filters, Raman filters, fish fluorescence filters, and in situ hybridization filters.
According to the application field, it can be divided into biological filters, astronomical filters, and medical filters
Characteristics of a good fluorescent filter:
1. Cut off depth deep
2. Low spontaneous fluorescence
3. The fluorescent filter has a good shape, which is conducive to the extraction of fluorescence imaging. If the surface smoothness is not good, wavefront torque or wedge angle will be generated, and wavefront torque will change the imaging position. The conventional transmitted wavefront torque has a more direct impact on imaging than the reflected wavefront torque
Fluorescent filters typically use a transparent glass substrate and are coated on both surfaces. The thickness of the anti reflection film will be selected as an integer multiple of half the wavelength, at which point the reflection superposition will be enhanced. The anti reflection thickness is a multiple of a quarter wavelength, and the superposition is enhanced at this time.
Fluorescent filters include various types of fluorescent filters, commonly used in microscopes to facilitate the observation of various fluorescent phenomena.
How to choose a fluorescent filter
The selection principle of fluorescence filters is to allow fluorescence (emitted light) to pass through the imaging end as much as possible and block excitation light to achieve high signal-to-noise ratio. Especially in the application of multiphoton excitation and total internal reflection microscopy, weak noise can cause significant interference to the imaging effect.
Usually, we study the transmission spectra of various parameters of fluorescent filters, but compared to the intensity of excitation light, fluorescence is often weak, so it is more necessary to refer to the blocking depth spectrum ODvalue to select fluorescent filters. Can intuitively reflect the shading characteristics of the filter.
The OD intersection point is an important criterion when selecting the excitation filter and the emission filter. Generally, ODvalue greater than 5 and ODvalue greater than OD6 are required to achieve better fluorescence signal-to-noise ratio (SNR).
Classification method of fluorescent filters:
According to different purposes, filters can be divided into TIRF filters, interference filters, total internal reflection filters, Raman filters, Raman filters, fish fluorescence filters, and in situ hybridization filters.
According to the application field, it can be divided into biological filters, astronomical filters, and medical filters
Characteristics of a good fluorescent filter:
1. Cut off depth deep
2. Low spontaneous fluorescence
3. The fluorescent filter has a good shape, which is conducive to the extraction of fluorescence imaging. If the surface smoothness is not good, wavefront torque or wedge angle will be generated, and wavefront torque will change the imaging position. The conventional transmitted wavefront torque has a more direct impact on imaging than the reflected wavefront torque