Introduction to the application of enzyme-linked immunosorbent assay (ELISA) filter
Enzyme linked immunosorbent assay (ELISA) is a specialized instrument used for reading and analyzing the results of enzyme-linked immunosorbent assay (ELISA). ELISA is performed by coupling enzyme catalyzed chromogenic substrates to antigens or antibodies. The reaction results are displayed in color. The concentration of antibodies or antigens in the sample can be determined by the depth of the colorimetric reaction, i.e. the absorbance value. With the development of detection methods, desktop enzyme-linked immunosorbent assay (ELISA) instruments with multiple detection methods are called multifunctional ELISA instruments, which can detect ABS, FI, TRF, FP, and LUM.
The enzyme-linked immunosorbent assay (ELISA) is generally divided into grating type and filter type. The grating enzyme-linked immunosorbent assay (ELISA) can capture any wavelength within the wavelength range of the light source, while the latter can only capture specific wavelengths based on the selected biochemical instrument interference filter.
Working principle of enzyme-linked immunosorbent assay (ELISA) reader:
The principle of enzyme-linked immunosorbent assay (ELISA) is to detect the absorbance of the analyte at a specific wavelength. The light wave emitted by the light source passes through the filter and becomes a monochromatic beam, which enters the sample and is tested in a plastic microporous rod. Part of it is absorbed, and the other part is irradiated onto the photodetector by the sample. The photodetector converts the light signals of different intensities of the sample into corresponding electrical signals. After signal processing such as pre amplification, logarithmic amplification, and analog-to-digital conversion, the electrical signal is sent to the microprocessor for data processing and calculation. Finally, the results are displayed on the monitor and printer.
Placement of filter for enzyme-linked immunosorbent assay:
When using a filter, like a regular colorimeter, the filter of an enzyme spectrometer can be placed in front of or behind a microplate. Or reach the reflector behind the spotlight. After 90 degree reflection on the mirror, the filter passes vertically through the colorimetric solution and is then sent to the photoelectric cell through the filter.
What does an enzyme-linked immunosorbent assay (ELISA) detect?
When light passes through the object being measured, the energy difference before and after detecting the object is the energy absorbed by the detected substance. At a specific wavelength, there is a quantitative relationship between the concentration and absorbed energy of the same detected object, represented by the OD value. OD stands for Optical Density, which represents the optical density absorbed by the substance being tested.
Factors influencing the test results:
Due to the many factors that affect the detection results, such as different enzyme-linked immunosorbent assay (ELISA) plates and the volume of detection reagents, different OD values can be obtained. Therefore, only by using the same ELISA plates and reaction reagents can the detection results be compared and analyzed.
The purpose of the enzyme-linked immunosorbent assay (ELISA) reader is to:
Applied to low ultraviolet region for reporter gene detection, G protein coupled receptor analysis, DNA, RNA quantification and purity analysis, protein quantification, enzyme activity, enzyme kinetics detection, cell apoptosis detection, enzyme-linked immunosorbent assay, cell proliferation and toxicity analysis, etc.
The enzyme-linked immunosorbent assay (ELISA) is generally divided into grating type and filter type. The grating enzyme-linked immunosorbent assay (ELISA) can capture any wavelength within the wavelength range of the light source, while the latter can only capture specific wavelengths based on the selected biochemical instrument interference filter.
Working principle of enzyme-linked immunosorbent assay (ELISA) reader:
The principle of enzyme-linked immunosorbent assay (ELISA) is to detect the absorbance of the analyte at a specific wavelength. The light wave emitted by the light source passes through the filter and becomes a monochromatic beam, which enters the sample and is tested in a plastic microporous rod. Part of it is absorbed, and the other part is irradiated onto the photodetector by the sample. The photodetector converts the light signals of different intensities of the sample into corresponding electrical signals. After signal processing such as pre amplification, logarithmic amplification, and analog-to-digital conversion, the electrical signal is sent to the microprocessor for data processing and calculation. Finally, the results are displayed on the monitor and printer.
Placement of filter for enzyme-linked immunosorbent assay:
When using a filter, like a regular colorimeter, the filter of an enzyme spectrometer can be placed in front of or behind a microplate. Or reach the reflector behind the spotlight. After 90 degree reflection on the mirror, the filter passes vertically through the colorimetric solution and is then sent to the photoelectric cell through the filter.
What does an enzyme-linked immunosorbent assay (ELISA) detect?
When light passes through the object being measured, the energy difference before and after detecting the object is the energy absorbed by the detected substance. At a specific wavelength, there is a quantitative relationship between the concentration and absorbed energy of the same detected object, represented by the OD value. OD stands for Optical Density, which represents the optical density absorbed by the substance being tested.
Factors influencing the test results:
Due to the many factors that affect the detection results, such as different enzyme-linked immunosorbent assay (ELISA) plates and the volume of detection reagents, different OD values can be obtained. Therefore, only by using the same ELISA plates and reaction reagents can the detection results be compared and analyzed.
The purpose of the enzyme-linked immunosorbent assay (ELISA) reader is to:
Applied to low ultraviolet region for reporter gene detection, G protein coupled receptor analysis, DNA, RNA quantification and purity analysis, protein quantification, enzyme activity, enzyme kinetics detection, cell apoptosis detection, enzyme-linked immunosorbent assay, cell proliferation and toxicity analysis, etc.