Principle of ELISA reader and application of ELISA filter
From the enzyme-linked immunosorbent assay, it can be seen that the enzyme-linked immunosorbent assay (ELISA) reader should use colorimetric method to analyze the antigen or antibody content. Speaking of which, an enzyme-linked immunosorbent assay (ELISA) reader is a disguised photoelectric colorimeter or spectrophotometer, and its working principle and main structure are basically similar to those of a photoelectric colorimeter.
The light emitted by the light source lamp passes through a filter or monochromatic device and becomes a monochromatic beam. The monochromatic beam passes through the sample in the plastic microplate, is absorbed by the sample, and then reaches the photodetector. The detector converts the intensity of the light signal projected onto it into the magnitude of an electrical signal. After pre amplification, logarithmic amplification, analog-to-digital conversion and other processing, the data is sent to the processor for data processing and calculation, and then the test results are displayed and printed by the monitor and printer.
The microprocessor also controls the movement of the mechanical drive mechanism in the X and Y directions through control circuits. However, for non automatic microplate readers, it is used to manually move the microplate, which can omit the mechanical drive mechanisms and circuits in the X and Y directions, making the instrument smaller in size and simpler in structure.
Microplate is a transparent plastic plate used to hold the sample to be tested. There are many rows of small holes on the board, such as 40, 55, and 96 small holes. Depending on the instrument, detection can be performed in one hole, one hole, and one hole.
The enzyme-linked immunosorbent assay (ELISA) can use a monochromator or or an interference filter to obtain monochromatic light. Like the photoelectric colorimeter, when using a filter as a filter device, the effect of filter design on the front and back of the microplate is the same.
The light emitted by the light source lamp passes through the spotlight and aperture to reach the reflector. After reflecting 90 degrees with a mirror, it vertically passes through the colorimetric solution and then enters the photovoltaic cell through a filter.
The beam of the enzyme-linked immunosorbent assay (ELISA) can be designed not only from top to bottom using colorimetric solutions, but also from bottom to top.
From the working principle diagram and optical path diagram of the enzyme-linked immunosorbent assay (ELISA) reader, it can be seen that the difference between it and a conventional photoelectric colorimeter is that: firstly, the container containing the colorimetric solution is not made of a colorimetric dish, but of a plastic microplate, which is made of a plastic microplate. Usually made of transparent polyethylene material. The reason for using plastic microplates as solid phase is that containers containing colorimetric solutions are made of plastic microplates. The characteristic of the carrier is that it has a strong adsorption effect on antigens or antibodies; The second is that the beam of the enzyme-linked immunosorbent assay (ELISA) passes through the liquid to be measured vertically; Thirdly, enzyme-linked immunosorbent assay (ELISA) usually uses optical density OD instead of A to represent absorbance.
The multi-channel fully automatic enzyme-linked immunosorbent assay (ELISA) reader is developed based on the single channel ELISA reader. Multi channel enzyme labeling is usually automated and equipped with multiple beams of light and photodetectors.
Ruicheng Optoelectronics supplies high-end narrowband filters that are fully suitable for various types of enzyme-linked immunosorbent assay (ELISA) analyzers.
The light emitted by the light source lamp passes through a filter or monochromatic device and becomes a monochromatic beam. The monochromatic beam passes through the sample in the plastic microplate, is absorbed by the sample, and then reaches the photodetector. The detector converts the intensity of the light signal projected onto it into the magnitude of an electrical signal. After pre amplification, logarithmic amplification, analog-to-digital conversion and other processing, the data is sent to the processor for data processing and calculation, and then the test results are displayed and printed by the monitor and printer.
The microprocessor also controls the movement of the mechanical drive mechanism in the X and Y directions through control circuits. However, for non automatic microplate readers, it is used to manually move the microplate, which can omit the mechanical drive mechanisms and circuits in the X and Y directions, making the instrument smaller in size and simpler in structure.
Microplate is a transparent plastic plate used to hold the sample to be tested. There are many rows of small holes on the board, such as 40, 55, and 96 small holes. Depending on the instrument, detection can be performed in one hole, one hole, and one hole.
The enzyme-linked immunosorbent assay (ELISA) can use a monochromator or or an interference filter to obtain monochromatic light. Like the photoelectric colorimeter, when using a filter as a filter device, the effect of filter design on the front and back of the microplate is the same.
The light emitted by the light source lamp passes through the spotlight and aperture to reach the reflector. After reflecting 90 degrees with a mirror, it vertically passes through the colorimetric solution and then enters the photovoltaic cell through a filter.
The beam of the enzyme-linked immunosorbent assay (ELISA) can be designed not only from top to bottom using colorimetric solutions, but also from bottom to top.
From the working principle diagram and optical path diagram of the enzyme-linked immunosorbent assay (ELISA) reader, it can be seen that the difference between it and a conventional photoelectric colorimeter is that: firstly, the container containing the colorimetric solution is not made of a colorimetric dish, but of a plastic microplate, which is made of a plastic microplate. Usually made of transparent polyethylene material. The reason for using plastic microplates as solid phase is that containers containing colorimetric solutions are made of plastic microplates. The characteristic of the carrier is that it has a strong adsorption effect on antigens or antibodies; The second is that the beam of the enzyme-linked immunosorbent assay (ELISA) passes through the liquid to be measured vertically; Thirdly, enzyme-linked immunosorbent assay (ELISA) usually uses optical density OD instead of A to represent absorbance.
The multi-channel fully automatic enzyme-linked immunosorbent assay (ELISA) reader is developed based on the single channel ELISA reader. Multi channel enzyme labeling is usually automated and equipped with multiple beams of light and photodetectors.
Ruicheng Optoelectronics supplies high-end narrowband filters that are fully suitable for various types of enzyme-linked immunosorbent assay (ELISA) analyzers.